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302.9: Identification of cytokine profiles in blood samples from kidney transplant recipients.
Зохиогч
Ariunaa Altangerel
Gansukh Chojilsuren
Ulziikhuu Tumurkhuyag
Enkhtushig Gavaabaljir
Sarantsetseg Jigjidsuren
Он
2024 оны есдүгээр сарын 1
Төрөл
journal article
Хэвлэгч
Wolters Kluwer
Journal
Transplantation
Volume
108
Issue
9S
ISSN
0041-1337
1534-6080
Хураангуй
Background: Cytokines, pivotal in the immune response following organ transplantation, serve as vital indicators of inflammation and guide immunosuppressive therapy. Precise cytokine analysis facilitates inflammation monitoring and immunosuppressive drug optimization kidney transplant recipients. Objective: This study aimed to delineate the cytokine profiles of kidney transplant patients pre and post transplantation utilizing flow cytometry. Materials and Methods: Conducted from July 2023 to March 2024, the study encompassed 10 kidney transplant recipients (5 male, 5 female; mean age 36±12.01 years) at the National University of Medical Sciences. Blood samples were collected before transplantation and at 7 and 30 days post surgery. Utilizing the Human Inflammation Panel 2, a 13-cytokine analysis was performed simultaneously employing the TC 13-plex (BioLegend®) bead-based multiplex assay panel, employing fluorescence-encoded beads suitable for flow cytometry. Statistical analyzes employed analysis of variance (ANOVA). Results: Evaluation of 13 cytokines (TGF-β1, PAI-1, sTREM-1, PTX3, sCD40L, sCD25, CXCL12, sST2, sTNF-RI, sTNF-RII, sRAGE, CXCL1, sCD130) revealed notable variations pre and post transplantation. Specifically, TGF-β1, sCD40L, sTNF-RII, and sRAGE exhibited elevated levels pre transplantation, which significantly decreased post transplantation (p<0.05). Conversely, the levels of other cytokines remained statistically unchanged pre and post transplantation (p>0.05). (Table 1)Conclusion: The study highlights a significant decrease (p<0.05) in TGF-β1, sCD40L, sTNF-RII, and sRAGE cytokine levels at 7 and 30 days post kidney transplantation, underscoring their potential as biomarkers for monitoring post transplant inflammatory responses. We would like to express our sincere gratitude to the following institutions for their support and collaboration throughout this study: Department of Immunology, Mongolian National University of Medical Sciences Institute of Biomedical Sciences, Mongolian National University of Medical Sciences Department of Clinical Laboratory, The First Central Hospital of Mongolia We extend our appreciation to the faculty, staff, and researchers at these institutions for their valuable contributions to this research project. Additionally, we thank the kidney transplant recipients who participated in this study for their cooperation and willingness to contribute to medical research.