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Antibacterial activity of Arthrobacter strains isolated from Great Gobi A Strictly Protected Area, Mongolia
Зохиогч
Bernacchi, Alberto
Semenzato, Giulia
di Mascolo, Manuel
Amata, Sara
Bechini, Angela
Berti, Fabiola
Calonico, Carmela
Catania, Valentina
Emiliani, Giovanni
Esposito, Antonia
Greco, Claudia
Mocali, Stefano
Mucci, Nadia
Padula, Anna
Piccionello, Antonio Palumbo
Nasanbat, Battogtokh
Davaakhuu, Gantulga
Riga, Francesco
Augugliaro, Claudio
Puglia, Anna Maria
Zaccaroni, Marco
Renato, Fani
Он
2024
Төрөл
journal article
Journal
AIMS microbiology
Volume
10
Issue
1
Start Page
161
End Page
186
ISSN
2471-1888
Хураангуй
Desert soil hosts many microorganisms, whose activities are essential from an ecological viewpoint. Moreover, they are of great anthropic interest. The knowledge of extreme environments microbiomes may be beneficial for agriculture, technology, and human health. In this study, 11 Arthrobacter strains from topsoil samples collected from the Great Gobi A Strictly Protected Area in the Gobi Desert, were characterized by a combination of different techniques. The phylogenetic analysis, performed using their 16S rDNA sequences and the most similar Arthrobacter sequences found in databases, revealed that most of them were close to A. crystallopoietes, while others joined a sister group to the clade formed by A. humicola, A. pascens, and A. oryzae. The resistance of each strain to different antibiotics, heavy-metals, and NaCl was also tested as well as the inhibitory potential against human pathogens (i.e., Burkholderia ssp., Klebsiella pneumoniae, Pseudomonas aeruginosa, and Staphylococcus ssp.) via cross-streaking, to check the production of metabolites with antimicrobial activity. Data obtained revealed that all strains were resistant to heavy metals and were able to strongly interfere with the growth of many of the human pathogens tested. The volatile organic compounds (VOCs) profile of the 11 Arthrobacter strains was also analyzed. A total of 16 different metabolites were found, some of which were already known for having an inhibitory action against different Gram-positive and Gram-negative bacteria. Isolate MS-3A13, producing the highest quantity of VOCs, is the most efficient against Burkholderia cepacia complex (Bcc), K. pneumoniae, and coagulase-negative Staphylococci (CoNS) strains. This work highlights the importance of understanding microbial populations' phenotypical characteristics and dynamics in extreme environments to uncover the antimicrobial potential of new species and strains.