Yandag, Munkhdelger

Товч нэр

Yandag, Munkhdelger

Бүтэн нэр

Yandag Munkhdelger

Латин нэр

Yandag Munkhdelger

Албан тушаал

Судлаач

Харьяалал

Анагаахын Шинжлэх Ухааны Үндэсний Их Сургууль

ORCID

0000-0003-0985-3491

Scopus Author ID

57193141947

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Detection and antibiotic resistance of diarrheagenic Escherichia coli from patients with diarrhea in Ulaanbaatar, Mongolia
(2023-02)
Yandag, Munkhdelger
;
Tsend-Ayush, Altansukh
;
Gunregjav, Nyamaa
;
Erdenebayar, Otgontsetseg
;
Byambadorj, Bayarlakh
INTRODUCTION: Diarrheal diseases are common with worldwide distribution, and diarrheagenic Escherichia coli (DEC) strains are the main causative agents. The present study aimed to define the association of various pathotypes of E. coli from diarrheal patients in Mongolia. METHODOLOGY: A total of 341 E. coli strains were isolated from the stool of diarrheal patients. Bacterial susceptibility to antimicrobial agents was determined by the Kirby Bauer disk diffusion method. DEC isolates were identified by HEp-2 cell adherence assay and multiplex polymerase chain reaction (PCR). RESULTS: DEC pathogens were detected in 53.7% of 341 E. coli isolates. Enteroaggregative E. coli (EAEC) was the most common DEC pathotype identified by HEp-2 adherence assay and multiplex PCR methods in 97 samples (28.4%), followed by atypical enteropathogenic E. coli (EPEC) in 50 samples (14.7%), diffusely adherent E. coli (DAEC) in 25 samples (7.3%), enterohaemorrhagic E. coli (EHEC) in 6 samples (1.8%), enterotoxigenic E. coli (ETEC) in 4 samples (1.2%), and enteroinvasive E. coli (EIEC) in 1 sample (0.3%). DEC strains had > 50% antibiotic resistance against cephalothin, ampicillin, and trimethoprim/sulfamethoxazole. All tested DEC strains were susceptible to imipenem. Among the 183 DEC strains, 27 (14.8%) were extended spectrum beta-lactamase producing isolates, and 125 (68.3%) isolates were multiple drug resistant. CONCLUSIONS: We have identified six pathotypes of DEC from the clinical isolates tested and concluded that a high prevalence of antimicrobial resistance was observed in these pathotypes. EAEC was the most common pathotype identified and this is the first report of EHEC identification in Mongolia.
Scopus© Citations 8
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Identification of Staphylococcus aureus Causing Contamination in Raw Beef and Meat-Processing Environments in Ulaanbaatar, Mongolia
(2025)
Dorjgochoo, Amgalanzaya
;
Batbayar, Anujin
;
Tsend-Ayush, Altansukh
;
Byambadorj, Bayarlakh
;
Jav, Sarantuya
Staphylococcus aureus (S. aureus) is a Gram-positive bacterium capable of causing a range of infections and displaying significant antibiotic resistance. S. aureus can exhibit resistance to multi-antibiotics, particularly penicillin, methicillin, linezolid, and daptomycin. The prevalence of methicillin-resistant S. aureus (MRSA) ranges from 10%-50% in China and Russia, neighboring countries of Mongolia. This study aimed to assess S. aureus contamination in raw beef samples and surface swabs from meat-processing areas and markets, while detecting, as well as to detect virulence and resistance genes in the isolates. A total of 156 raw beef samples and 131 surface swabs were collected and analyzed using ISO 6888-1:2021 standards. The nucA gene specific to S. aureus was amplified by PCR, and antibiotic susceptibility was evaluated using the Kirby-Bauer disk diffusion method. Resistance genes (mecA, mecC, vanA, and vanB) and virulence genes (sea, sed, tsst, eta, and etb) were detected via PCR. The results showed contamination rates of 26.9% in raw beef and 15.3% in surface swabs. The isolates exhibited high resistance to oxacillin, ampicillin, and penicillin in meat samples and to oxacillin, tetracycline, azithromycin, and clindamycin in surface swabs. No resistance genes for vancomycin or methicillin (mecC, vanA, vanB) were detected. Virulence genes, including tsst (14.5%), sea and etb (9.7%), eta (3.2%), and sed (1.6%), were identified. Contamination was more prevalent in centers responsible for both transportation and sales, compared to meat-processing areas. These findings highlight the need for stricter hygiene and handling practices in meat transport and markets to reduce S. aureus contamination and limit the spread of resistant strains.
Scopus© Citations 1
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Detection of virulence genes, phylogenetic group and antibiotic resistance of uropathogenic Escherichia coli in Mongolia
(2017-01)
Munkhdelger, Yandag
;
Gunregjav, Nyamaa
;
Dorjpurev, Altantsetseg
;
Juniichiro, Nishi
;
Sarantuya, Jav
INTRODUCTION: The severity of urinary tract infection (UTI) produced by uropathogenic Escherichia coli (UPEC) is due to the expression of a wide spectrum of virulence genes. E. coli strains were divided into four phylogenetic groups (A, B1, B2 and D) based on their virulence genes. The present study aimed to assess the relationship between virulence genes, phylogenetic groups, and antibiotic resistance of UPEC. METHODOLOGY: A total of 148 E. coli were tested for antimicrobial resistance against 10 drugs using the disk diffusion method. The isolates were screened by polymerase chain reaction (PCR) for detection of virulence genes and categorized into the four major phylogenetic groups. RESULTS: Phylogenetic group B2 was predominant (33.8%), followed by D (28.4%), A (19.6), and B1 (18.2%). A higher prevalence of fimH (89.9%), fyuA (70.3%), traT (66.2%), iutA (62.2%), kpsMTII (58.8%), and aer (56.1%) genes were found in UPEC, indicating a putative role of adhesins, iron acquisition systems, and protectins that are main cause of UTIs. The most common antibiotic resistance was to cephalotin (85.1%), ampicillin (78.4%) and the least to nitrofurantoin (5.4%) and imipenem (2%). In total, 93.9% of isolates were multidrug resistant (MDR). CONCLUSIONS: This study showed that group B2 and D were the predominant phylogenetic groups and virulence-associated genes were mostly distributed in these groups. The virulence genes encoding components of adhesins, iron acquisition systems, and protectins were highly prevalent among antibiotic-resistant UPEC. Although the majority of strains are MDR, nitrofurantoin is the drug of choice for treatment of UTI patients in Ulaanbaatar.
Scopus© Citations 78
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Detection of virulence genes of Staphylococcus aureus isolated from raw beef for retail sale in the markets of Ulaanbaatar city, Mongolia
(2023-11)
Dorjgochoo, Amgalanzaya
;
Batbayar, Anujin
;
Tsend-Ayush, Altansukh
;
Erdenebayar, Otgontsetseg
;
Byambadorj, Bayarlakh
BACKGROUND: Staphylococcus aureus (S. aureus) is a highly virulent pathogen that causes food-borne illness, food poisoning, skin and soft tissue infections, abscesses, mastitis, and bacteremia. It is common for meat and meat products to become contaminated with S. aureus due to dirty hands, food storage conditions, food production processes, and unhygienic conditions, causing food poisoning. Therefore, we aimed to isolate S. aureus strain from the raw beef and reveal virulence genes and antibiotic resistance profile from isolated S. aureus strains. METHODS: In this study, 100 samples of raw beef were collected from 4 major market stalls in Ulaanbaatar city, Mongolia. S. aureus was detected according to the ISO 6888-1:2021 standard, and the nucA gene encoding the species-specific thermonuclease was amplified and confirmed by polymerase chain reaction (PCR). In the strains of S. aureus isolated from the samples, the genes encoding the virulence factors including sea, sed, tsst, eta, etb, and mecA were amplified by multiplex PCR. These genes are encoded staphylococcal enterotoxin A, enterotoxin D, toxic shock syndrome toxin, exotoxin A, exotoxin B and penicillin-binding protein PBP 2A, respectively. Antibiotic sensitivity test was performed by the Kirby-Bauer disc diffusion method. The Clinical and Laboratory Standard Institute guidelines as CLSI M100-S27 was used for analysis of the data. RESULTS: Thirty-five percent of our samples were detected contaminated with of the S. aureus strains. Subsequently, antibiotic resistance was observed in the S. aureus contaminated samples. Among our samples, the highest rates of resistance were determined against ampicillin (97.1%), oxacillin (88.6%), and penicillin (88.6%), respectively. Three genes including mecA, sea, and tsst from six virulence genes were detected in 17% of S. aureus strain-contaminated samples by multiplex PCR. The sed, etb and eta genes were detected in the 2.9%, 11.4% and 5.7% of our samples, respectively. CONCLUSION: The results show that S. aureus related contamination is high in the raw beef for retail sale and prevalent S. aureus strains are resistant to all antibiotics used. Also, our results have demonstrated that there is a high risk for food poisoning caused by antibiotic resistant S. aureus in the raw beef and it may establish public health issues. Genes encoding for both heat-resistant and nonresistant toxicity factors were detected in the antibiotic resistant S. aureus strains and shown the highly pathogenic. Finally, our study is ensuring to need proper hygienic conditions during beef's preparation and sale.
Scopus© Citations 16

Yandag, Munkhdelger

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